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KMID : 0988920130110040261
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Intestinal Research
2013 Volume.11 No. 4 p.261 ~ p.267
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Epigallocatechin-3-gallate Inhibits the Expression of Adhesion Molecules by Blocking Nuclear Factor Kappa B Signaling in Intestinal Epithelial Cells
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Myung Dae-Seong
Park Young-Lan
Joo So-Young
Myung Eun
Chung Cho-Yun
Park Hyung-Chul
Kim Jong-Sun
Cho Sung-Bum
Lee Wan-Sik
Kim Hyun-Soo
Joo Young-Eun
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Abstract
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Background/Aims: Epigallocatechin-3-gallate (EGCG) is the main polyphenol in green tea and has anti-inflammatory and anti-oxidative effects. The aim of this study was to determine the impact of EGCG on the expression of adhesion molecules and lipopolysaccharide (LPS)-induced nuclear factor-kappa B (NF-?B) signaling in rat intestinal epithelial (RIE) cells.
Methods: The effect of EGCG on LPS-induced NF-?B signaling and expression of intercellular adhesion molecule (ICAM)-1 and vascular cell adhesion molecule (VCAM)-1 was examined by reverse transcription polymerase chain reaction, western blotting, immunofluorescence and electrophoretic mobility shift assay.
Results: LPS-induced expression of ICAM-1 and VCAM-1 mRNA was inhibited by EGCG treatment in RIE cells. LPS-induced inhibitor of kappa B alpha degradation and NF-?B nuclear translocation were blocked by EGCG in RIE cells. EGCG blocked LPS-induced NF-?B DNA-binding activity in RIE cells. The pharmacological NF-?B inhibitor Bay11-7082 suppressed the LPS-induced expression of ICAM-1 and VCAM-1 mRNA in RIE cells.
Conclusions: These results indicate that EGCG inhibits LPS-induced ICAM-1 and VCAM-1 expression by blocking NF-?B signaling in intestinal epithelial cells.
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KEYWORD
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Epigallocatechin gallate, Nuclear factor kappa B, Adhesion molecule, Intestinal epithelial cell
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